From 86b1b192501eecc8a4e566795327772c32b1633d Mon Sep 17 00:00:00 2001 From: Justin Bedo Date: Wed, 5 Dec 2018 10:48:05 +1100 Subject: bowtie: init --- tools/bowtie-align.nix | 36 ++++++++++++++++++++++++++++++++++++ 1 file changed, 36 insertions(+) create mode 100644 tools/bowtie-align.nix (limited to 'tools/bowtie-align.nix') diff --git a/tools/bowtie-align.nix b/tools/bowtie-align.nix new file mode 100644 index 0000000..456747a --- /dev/null +++ b/tools/bowtie-align.nix @@ -0,0 +1,36 @@ +{ bionix +, nixpkgs +, ref +, bamOutput ? true +, flags ? null +, indexAttrs ? {} +}: + +{ input1 +, input2 ? null +}: + +with nixpkgs; +with lib; +with bionix.types; +with bionix.compression; + +let + fa = f: matchFiletype "bowtie2-ref" { fa = _: f; } f; + fq = f: matchFiletype "bowtie2-input" { fq = _: f; gz = matchFiletype' "bowtie2-input" { fq = _: f; }; } f; + +in stdenv.mkDerivation { + name = "bowtie2-align"; + buildInputs = [ bowtie2 bc ] ++ optional bamOutput samtools; + buildCommand = '' + cores=$(echo $NIX_BUILD_CORES ${optionalString bamOutput "- 1"} | bc) + if [[ $cores -lt 1 ]] ; then + cores=1 + fi + bowtie2 -x ${bionix.bowtie.index indexAttrs ref}/ref ${optionalString (flags != null) flags} --threads $cores \ + ${if input2 != null then "-1 " + fq input1 + " -2 " + fq input2 else "-U " + fq input1} \ + ${optionalString bamOutput "| samtools view -b"} \ + > $out + ''; + passthru.filetype = if bamOutput then filetype.bam {ref = ref; sorting = sort.name {};} else filetype.sam {ref = ref; sorting = sort.name {};}; +} -- cgit v1.2.3