{ bionix , ref , bamOutput ? true , flags ? null , indexAttrs ? {} }: { input1 , input2 ? null }: with bionix; with lib; with types; with compression; let fa = f: matchFiletype "bowtie2-ref" { fa = _: f; } f; fq = f: matchFiletype "bowtie2-input" { fq = _: f; gz = matchFiletype' "bowtie2-input" { fq = _: f; }; } f; in stage { name = "bowtie2-align"; buildInputs = with pkgs; [ bowtie2 bc samtools ]; buildCommand = '' cores=$(echo $NIX_BUILD_CORES ${optionalString bamOutput "- 1"} | bc) if [[ $cores -lt 1 ]] ; then cores=1 fi bowtie2 -x ${bionix.bowtie.index indexAttrs ref}/ref ${optionalString (flags != null) flags} --threads $cores \ ${if input2 != null then "-1 " + fq input1 + " -2 " + fq input2 else "-U " + fq input1} \ | samtools sort -n \ ${optionalString bamOutput "| samtools view -b"} \ > $out ''; passthru.filetype = if bamOutput then filetype.bam {ref = ref; sorting = sort.name {};} else filetype.sam {ref = ref; sorting = sort.name {};}; passthru.multicore = true; }