{ bionix , ref , bamOutput ? true , flags ? null , indexAttrs ? {} }: { input1 , input2 ? null }: with bionix; with lib; with types; with compression; let fa = f: matchFiletype "bwa-ref" { fa = _: f; } f; fq = f: matchFiletype "bwa-input" { fq = _: f; gz = matchFiletype' "bwa-input" { fq = _: f; }; } f; in stage { name = "bwa-mem2"; buildInputs = with pkgs; [ bionix.bwa.app2 bc ] ++ optional bamOutput samtools; buildCommand = '' ln -s ${fa ref} ref.fa for f in ${bionix.bwa.index2 indexAttrs ref}/* ; do ln -s $f done cores=$(echo $NIX_BUILD_CORES ${optionalString bamOutput "- 1"} | bc) if [[ $cores -lt 1 ]] ; then cores=1 fi bwa-mem2 mem ${optionalString (flags != null) flags} -t $cores ref.fa ${fq input1} \ ${optionalString (input2 != null) (fq input2)} \ ${optionalString bamOutput "| samtools view -b"} \ > $out ''; passthru.filetype = if bamOutput then filetype.bam {ref = ref; sorting = sort.name {};} else filetype.sam {ref = ref; sorting = sort.name {};}; passthru.multicore = true; }