{ bionix , ref , bamOutput ? true , flags ? null , indexAttrs ? {} }: { input1 , input2 ? null }: with bionix; with lib; with types; with compression; let fa = f: matchFiletype "hisat2-ref" { fa = _: f; } f; fq = f: matchFiletype "hisat2-input" { fq = _: f; gz = matchFiletype' "hisat2-input" { fq = _: "<(gunzip < ${f})"; }; } f; in stage { name = "hisat2-align"; buildInputs = with pkgs; [ hisat2 bc samtools ]; buildCommand = '' ln -s ${fa ref} ref.fa cores=$(echo $NIX_BUILD_CORES ${optionalString bamOutput "- 1"} | bc) if [[ $cores -lt 1 ]] ; then cores=1 fi hisat2 ${optionalString (flags != null) flags} -p $cores -x ${hisat2.index indexAttrs ref}/index \ ${if input2 != null then "-1 ${fq input1} -2 ${fq input2}" else "-U ${fq input1}"} \ ${optionalString bamOutput "| samtools view -b"} \ | samtools sort -n \ > $out ''; passthru.filetype = if bamOutput then filetype.bam {ref = ref; sorting = sort.name {};} else filetype.sam {ref = ref; sorting = sort.name {};}; passthru.multicore = true; }