{ bionix , ref , bamOutput ? true , flags ? null , preset , RG ? { } }: { input1 , input2 ? null }: with bionix; with lib; with types; with compression; let fa = f: matchFiletype "minimap2-ref" { fa = _: f; } f; fq = f: matchFiletype "minimap2-input" { fq = _: f; gz = matchFiletype' "minimap2-input" { fq = _: f; }; } f; in stage { name = "minimap2-align"; buildInputs = with pkgs; [ minimap2 bc ] ++ optional bamOutput samtools; buildCommand = '' ln -s ${fa ref} ref.fa cores=$(echo $NIX_BUILD_CORES ${optionalString bamOutput "- 1"} | bc) if [[ $cores -lt 1 ]] ; then cores=1 fi minimap2 ${optionalString (flags != null) flags} -t $cores -ax ${preset} ref.fa ${fq input1} \ ${optionalString (input2 != null) (fq input2)} \ ${optionalString (RG ? ID) "-R'@RG\\t${concatMapAttrsStringsSep "\\t" (k: v: "${k}:${v}") RG}'"} \ ${optionalString bamOutput "| samtools view -b"} \ > $out ''; passthru.filetype = if bamOutput then filetype.bam { inherit ref; sorting = sort.none { }; } else filetype.sam { inherit ref; sorting = sort.none { }; }; passthru.multicore = true; }