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|
{ bionix
, nixpkgs
, blacklist ? null
, bwaIndexAttrs ? {}
, faidxAttrs ? {}
, flags ? null
, config ? null
}:
with nixpkgs;
with lib;
with bionix.types;
inputs:
let
getref = matchFiletype "gridss-callVariants" { bam = x: x.ref; };
refs = map getref inputs;
ref = head refs;
in
assert (length (unique refs) == 1);
stdenv.mkDerivation rec {
name = "gridss-callVariants";
buildInputs = [ jre R bwa ];
buildCommand = ''
ln -s ${ref} ref.fa
ln -s ${bionix.samtools.faidx faidxAttrs ref} ref.fa.fai
for f in ${bionix.bwa.index bwaIndexAttrs ref}/*; do
ln -s $f
done
mkdir $out
java -ea -Xmx31g \
-Dreference_fasta="ref.fa" \
-Dsamjdk.create_index=true \
-Dsamjdk.use_async_io_read_samtools=true \
-Dsamjdk.use_async_io_write_samtools=true \
-Dsamjdk.use_async_io_write_tribble=true \
-Dgridss.gridss.output_to_temp_file=true \
-cp ${bionix.gridss.jar} gridss.CallVariants \
WORKER_THREADS=$NIX_BUILD_CORES \
TMP_DIR=. \
WORKING_DIR=. \
${optionalString (config != null) ("CONFIGURATION_FILE=" + bionix.gridss.gridssConfig config)} \
REFERENCE_SEQUENCE="ref.fa" \
${concatMapStringsSep " " (i: "INPUT=\"${i}\"") inputs} \
OUTPUT="$out/gridss.vcf" \
ASSEMBLY="$out/gridss.bam" \
${optionalString (blacklist != null) ("BLACKLIST=" + blacklist)} \
${optionalString (flags != null) flags}
'';
}
|
