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|
{ bionix
, ref
, bamOutput ? true
, flags ? null
, preset
}:
{ input1
, input2 ? null
}:
with bionix;
with lib;
with types;
with compression;
let
fa = f: matchFiletype "minimap2-ref" { fa = _: f; } f;
fq = f: matchFiletype "minimap2-input" { fq = _: f; gz = matchFiletype' "minimap2-input" { fq = _: f; }; } f;
in stage {
name = "minimap2-align";
buildInputs = with pkgs; [ minimap2 bc ] ++ optional bamOutput samtools;
buildCommand = ''
ln -s ${fa ref} ref.fa
cores=$(echo $NIX_BUILD_CORES ${optionalString bamOutput "- 1"} | bc)
if [[ $cores -lt 1 ]] ; then
cores=1
fi
minimap2 ${optionalString (flags != null) flags} -t $cores -ax ${preset} ref.fa ${fq input1} \
${optionalString (input2 != null) (fq input2)} \
${optionalString bamOutput "| samtools view -b"} \
> $out
'';
passthru.filetype = if bamOutput then filetype.bam {ref = ref; sorting = sort.none {};} else filetype.sam {ref = ref; sorting = sort.none {};};
passthru.multicore = true;
}
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